Development of Bone regeneration therapy by undifferentiated osteoblast transplantation

Tissue Engineering technology combining human osteoblasts isolated from human jawbone and 3-Dimensional scaffold to regenerate horizontal defects caused by severe periodontitis. This project is jointly developed with the National Institute of Advanced Industrial Science and Technology. We are focused on research and development of medical health care products.

Investigation of target molecules in the treatment resistant apical periodontitis cases

　We are investigating how treatment resistant apical periodontitis cases were induced in patient with systeic diseases, and develop novel anti-inflammatory therapy to overcome these problems.

Development of new root canal cleaning technology

In this project, we are investigating the efficacy of various root canal irrigation methods for the complete removal of intracanal biofilm to provide a novel disinfection method for apical periodontitis.

Application of Concentrated growth Factors (CGF) as anti-inflammatory therapy for Apical periodontitis

In collaboration with Tokyo Medical and Dental University, Osaka University, and Niigata University, we are using CGF following Endodontic surgery to enhance wound healing. The CGF are obtained from patients’ blood just before surgery and it contains significant amount various growth factors that stimulate resident stem cells to accelerate periapical tissue healing.

Development of oral management technology applying IoT and saliva test

We are developing a new monitoring system that evaluates disease activity from the oral region using inspecting technology that applies IoT technology to intractable diseases such as Marfan syndrome, inflammatory bowel disease, and diabetes.

Research achievement

  1. Suresh V. V, Handa K, Njuguna M, Hasegawa T, Maruyama K, Nemoto E, Yamada S, Sugawara S, Lu L, Takedachi M, Murakami S, Okura H, Matsuyama A & Saito M *: Periodontal Regeneration by Allogeneic Transplantation of Adipose Tissue Derived Multi- Lineage Progenitor Stem Cells in vivo. Sci Rep. 2019 Jan 29;9(1):921. doi: 10.1038/s41598-018-37528-0.
  2. Handa K, Abe S, Suresh VV, Fujieda Y, Ishikawa M, Orimoto A, Kobayashi Y, Yamada S, Yamaba S, Murakami S, Saito M. Fibrillin-1 insufficiency alters periodontal wound healing failure in a mouse model of Marfan syndrome. Arch Oral Biol. 2018 Mar 6;90:53-60. doi: 10.1016/j.archoralbio.2018.02.017.
  3. Iwamatsu-Kobayashi Y, Abe S, Fujieda Y, Orimoto A, Kanehira M, Handa K, Venkataiah V S, Zou W, Ishikawa M, and Saito M. Metal ions from S-PRG filler have the potential to prevent periodontal disease Clinical and Experimental Dental Research Volume 3, Issue 4 August 2017 Pages 126–133.
  4. Orimoto A, Kurokawa M, Handa K, IshikawaM, Nishida E, Aino M, MitaniA, Ogawa M, Tsuji T Saito M, F-spondin negatively regulates dental follicle differentiation through the inhibition of TGF-b activity. Arch Oral Biol. 2017 Mar 1;79:7-13. doi:10.1016/j.archoralbio.2017.02.019.
  5. Umeyama K, Watanabe K, Watanabe M, Horiuchi K, Nakano K, Kitashiro M, Matsunari H, Kimura T, Arima Y, Sampetrean O, Nagaya M, Saito M, Saya H, Kosaki K, Nagashima H & Matsumoto M, Generation of heterozygous fibrillin-1 mutant cloned pigs from genome-edited foetal fibroblasts. Sci Rep. 2016 April 14;6:24413. DOI:10.1038/srep24413
  6. Aino M, Nishida E, Fujieda Y, Orimoto A, Mitani A, Noguchi T, Makino H, Murakami S, Umezawa A, Yoneda T, Saito M. Isolation and characterization of the human immature osteoblast culture system from the alveolar bones of aged donors for bone regeneration therapy. Expert Opin Biol Ther. 2014 Dec;14(12):1731-44. doi:10.1517/14712598.2014.960387. Epub 2014 Sep 22
  7. Saito K, Fukumoto E, Yamada A, Yuasa K, Yoshizaki K, Iwamoto T, Saito M, Nakamura T, Fukumoto S. Interaction between Fibronectin and β1 Integrin Is Essential for Tooth Development. PLoS One. 2015 Apr 1;10(4):e0121667. doi:10.1371/journal.pone.0121667

Performance list

  1. Masahiro SAITO, Periodontal tissue regeneration ability of adipose tissue derived multilineage progenitor cells upon allogeneic transplantation, IOHS, Fozhou, 2019.1.11
  2. Masahiro SAITO, V. Venkata Suresh, Keisuke HANDA, Masahide TAEKDACHI, Hanayuki OKURA, Akifumi MATSUYAMA and Shinya MURAKAMI, Periodontal tissue regeneration ability of adipose tissue derived multilineage progenitor cells upon allogeneic transplantation, TERMIS, Kyoto, 2018.9.6
  3. 齋藤正寛　口腔と全身の疾患を繋げる共通の標的：メカノバイオロジーを介した結合組織疾患発症機構の解析　第147回日本歯科保存学会秋季学術大会平成29年10月27日盛岡
  4. Masahiro Saito Role of ADAMTSL6 on aortic aneurysm and dissection in Marfan syndrome Gordon reserch conference Elastin, Elastic fibers & microfibrils. 2017.8.1 Boston
  5. 齋藤正寛　歯周組織再生医療の開発と課題　第16回日本再生医療学会総会　2017年3月7日仙台
  6. Orimoto A, Murasawa Y, Isogai Z, Chijiiwa M, Mochizuki S, Imanaka- Yoshida K, Okada Y and Saito M, ADAMTSL6exacerbates tissue destruction of aortic aneurysm and dissection in Marfan syndrome mouse model through promotion of ADAMTS4 activity. ASMB2016, St. Petersburg, 2016.11.15
  7. Masahiro Saito, Current strategy for periodontal regenerative therapy. Chulalongkorn-Tohoku joint symposium in dental science 2015, Bankok, 2015,12.9
  8. Current strategies for bone regeneration therapy in the treatment of periodontal disease. K. Handa, V. Suresh, M. Ishikawa, and M. Saito, Eight French Reserch Organization-Tohoku University Joint Workshop on Frontier Materials,Sendai, 2015.12.2
  9. 齋藤正寛、細胞外マトリックス再生を導く新規創薬の開発　〜歯からはじまり大動脈へ〜、第13回日本再生歯科医学会学術大会・総会、新潟、2015.8.29
  10. 齋藤正寛、東北連携を基盤とした歯科再生医療の開発、臨床歯周病学会第33回年次大会、仙台、2015.7.19
  11. 齋藤正寛、細胞外マトリックス補充療法による新規再生医療技術の開発、抗加齢学会第15回総会、2015.5.29

Review paper

  1. 長谷川達也、中野将人、鈴木重人、半田慶介、八幡祥生、佐藤暢也、齋藤正寛：根尖性歯周炎の免疫学と治療抵抗性に対する新規歯内療法開発への課題、日本歯内療法学会誌 41(1):8-15, 2020
  2. 齋藤正寛：口腔と全身の疾患を繋げる共通の標的：メカノバイオロジーを介した結合組織疾患発症機構の解析　日本歯科保存学雑誌 61(2):85-89, 2018
  3. 齋藤正寛　歯科領域における再生医療の最前線　日本歯科医師会雑誌66(9)、21-30, 2013

Book

  1. 齋藤 正寛、半田 慶介：第２章歯科再生学に関わる細胞外基質、歯科再生学に関わる幹細胞、歯科再生医学、医歯薬出版、村上 伸也、網塚 憲生、齋藤 正寛、松本 卓也　編集、ISBN: 978-4-263-45838-9(2019年4月)P112-137
  2. 半田 慶介、齋藤 正寛：第２章作業長決定の重要性、マストオブイニシャルトリートメント、デンタルダイヤモンド、北村 和夫 編著、ISBN: 978-4-88510-414-5(2018年8月)P84-87
  3. 半田 慶介、齋藤 正寛：第８章歯周組織再生療法の実用化にむけた技術開発と課題、歯科再生・修復医療と材料、シーエムシー出版（2015年8月）P72-81

Bulletin

  1. 八幡祥生、齋藤正寛、根管洗浄技術の基本概念と術式の進歩、別冊 the Quintessence「歯内療法のパラダイムシフト」 Page88-93(2020年6月)
  2. 半田 慶介、齋藤 正寛、文献と臨床の橋わたし　MSCによる再生医療の戦略とその意義(第3回)　MSCによる他家移植の未来予想図、日本歯科評論78巻6号 Page151-154(2018年6月)
  3. 半田 慶介、齋藤 正寛、文献と臨床の橋わたし　MSCによる再生医療の戦略とその意義(第2回)　MSCによる自家移植再生療法の最前線、日本歯科評論78巻5号 Page155-158(2018年5月)
  4. 半田 慶介、齋藤 正寛、文献と臨床の橋わたし　MSCによる再生医療の戦略とその意義(第1回)　MSCによる再生医療の概略、日本歯科評論78巻4号 Page149-152(2018年4月)
  5. 半田 慶介、齋藤正寛、歯周組織における再生療法の最前線と実用化への課題、PHARMSTAGE、2017年10月号、ISDN:1346-3918
  6. 半田 慶介、相野誠、三谷章雄、齋藤正寛、未分化骨芽細胞を用いた新規骨再生医療技術の開発、別冊クインテセンス、口腔外科ハンドマニュアル15,161-166(2015)